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multi-analyte elisarray kit  (Qiagen)


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    Qiagen multi-analyte elisarray kit
    Multi Analyte Elisarray Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multi-analyte+elisarray+kit/multi+analyte+elisarray+kit/pmc12192584-442-14-17
    Average 90 stars, based on 1 article reviews
    multi-analyte elisarray kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Cytokine Assay:

    Article Title: Antioxidant, antimicrobial and immunomodulatory activities of facial serum formulation containing Melaleuca cajuputi essential oil
    Article Snippet: The percentage of viability was calculated by using the following formula (Lin and Lin, 2020): Viability (%) = (ODtreated cells/ODuntreated cells) × 100 Cytokine assay For the detection of mouse T helper 1 (Th1), T helper 2 (Th2) and T helper 17 (Th17) related cytokines, cytokine assay was performed using a multi-analyte ELISArray kit (QIAGEN, USA) according to the manufacturer’s instructions with cell culture supernatants as samples.

    Article Title: Translation of Data from Animal Models of Cancer to Immunotherapy of Breast Cancer and Chronic Lymphocytic Leukemia
    Article Snippet: Cytokine measurements in vitro/in serum : A Multi-Analyte ELISArray kit (Qiagen, Venlo, The Netherlands) was used to check multiple cytokines in the culture supernatant of BMMSCs or CLL cells alone, or in co-culture.

    Article Title: Protective effects of Centella asiatica extract on spatial memory and learning deficits in animal model of systemic inflammation induced by lipopolysaccharide.
    Article Snippet: On day 21 after the behavioral test, all rats were euthanized and blood was obtained through heart puncture into eDta-coated tubes. samples were centrifuged (6000 g for 15 min at 4 °c) and plasma was collected and stored frozen (–80 °c) until analysis. the plasma cytokines were measured using a Multi-analyte elisarray kit (cat. no. MeR-004a; saBiosciences; Qiagen, inc, Valencia, ca, Usa) according to the manufacturer’s protocol. samples were added to the array, which included the following specific cytokine capture antibodies from the above-mentioned kit: il-1β, il-6, tNf-α, ifN-γ and McP-1. the reaction was measured by changes at 450 and 540 nm on a spectrophotometer.

    Article Title: Anti-Inflammatory Action of Resveratrol in the Central Nervous System in Relation to Glucose Concentration—An In Vitro Study on a Blood–Brain Barrier Model
    Article Snippet: IL-6, IL-8, with the Multi-Analyte ELISArray Kit (Qiagen-Q4Lab, Hilden, Germany; cat. # 336161), according to the instructions of the manufacturer.

    Article Title: Proinflammatory cytokine profiles in prediabetic Saudi patients
    Article Snippet: The Multi-Analyte ELISArray Kit was used to analyze the presence of 12 human cytokines associated with inflammation in plasma samples from prediabetes patients and healthy controls (Qiagen).

    Article Title: Regulation of distinct caspase-8 functions in retinal ganglion cells and astroglia in experimental glaucoma
    Article Snippet: Protein lysates of the retina were obtained by homogenization in a lysis buffer (50 mM HEPES-KOH pH 8.0, 100 mM KCl, 2 mM EDTA, 0.10% NP-40, 2 mM dithiothreitol, 10% glycerol, supplemented with protease and phosphatase inhibitors) as previously described. ( ; ) After protein concentration was determined using a colorimetric Bradford protein assay (BioRad, Hercules, CA), a set of cytokines/chemokines was profiled, as previously described ( ; ), using a Multi-Analyte ELISArray kit (Qiagen, Valencia, CA) with detection sensitivity at low pg/ml levels.

    Article Title: Quercetin as a Therapeutic Option in a Rat Model of Aluminum Chloride- and D-Galactose-Induced Neurodegeneration
    Article Snippet: The levels of pro-inflammatory cytokines and chemokines in brain samples were determined using a Multi-Analyte ELISArray Kit (Qiagen, Hilden, Germany).

    Article Title: Impact of Epstein-Barr Virus Nuclear Antigen 1 on Neuroinflammation in PARK2 Knockout Mice.
    Article Snippet: Cytokine concentrations in the culture supernatants were then measured using a multi-analyte ELISArray kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions.

    Cell Culture:

    Article Title: Antioxidant, antimicrobial and immunomodulatory activities of facial serum formulation containing Melaleuca cajuputi essential oil
    Article Snippet: The percentage of viability was calculated by using the following formula (Lin and Lin, 2020): Viability (%) = (ODtreated cells/ODuntreated cells) × 100 Cytokine assay For the detection of mouse T helper 1 (Th1), T helper 2 (Th2) and T helper 17 (Th17) related cytokines, cytokine assay was performed using a multi-analyte ELISArray kit (QIAGEN, USA) according to the manufacturer’s instructions with cell culture supernatants as samples.

    Article Title: Translation of Data from Animal Models of Cancer to Immunotherapy of Breast Cancer and Chronic Lymphocytic Leukemia
    Article Snippet: Cytokine measurements in vitro/in serum : A Multi-Analyte ELISArray kit (Qiagen, Venlo, The Netherlands) was used to check multiple cytokines in the culture supernatant of BMMSCs or CLL cells alone, or in co-culture.

    Article Title: Protective effects of Centella asiatica extract on spatial memory and learning deficits in animal model of systemic inflammation induced by lipopolysaccharide.
    Article Snippet: On day 21 after the behavioral test, all rats were euthanized and blood was obtained through heart puncture into eDta-coated tubes. samples were centrifuged (6000 g for 15 min at 4 °c) and plasma was collected and stored frozen (–80 °c) until analysis. the plasma cytokines were measured using a Multi-analyte elisarray kit (cat. no. MeR-004a; saBiosciences; Qiagen, inc, Valencia, ca, Usa) according to the manufacturer’s protocol. samples were added to the array, which included the following specific cytokine capture antibodies from the above-mentioned kit: il-1β, il-6, tNf-α, ifN-γ and McP-1. the reaction was measured by changes at 450 and 540 nm on a spectrophotometer.

    Article Title: Anti-Inflammatory Action of Resveratrol in the Central Nervous System in Relation to Glucose Concentration—An In Vitro Study on a Blood–Brain Barrier Model
    Article Snippet: IL-6, IL-8, with the Multi-Analyte ELISArray Kit (Qiagen-Q4Lab, Hilden, Germany; cat. # 336161), according to the instructions of the manufacturer.

    Article Title: Proinflammatory cytokine profiles in prediabetic Saudi patients
    Article Snippet: The Multi-Analyte ELISArray Kit was used to analyze the presence of 12 human cytokines associated with inflammation in plasma samples from prediabetes patients and healthy controls (Qiagen).

    Article Title: Regulation of distinct caspase-8 functions in retinal ganglion cells and astroglia in experimental glaucoma
    Article Snippet: Protein lysates of the retina were obtained by homogenization in a lysis buffer (50 mM HEPES-KOH pH 8.0, 100 mM KCl, 2 mM EDTA, 0.10% NP-40, 2 mM dithiothreitol, 10% glycerol, supplemented with protease and phosphatase inhibitors) as previously described. ( ; ) After protein concentration was determined using a colorimetric Bradford protein assay (BioRad, Hercules, CA), a set of cytokines/chemokines was profiled, as previously described ( ; ), using a Multi-Analyte ELISArray kit (Qiagen, Valencia, CA) with detection sensitivity at low pg/ml levels.

    Article Title: Quercetin as a Therapeutic Option in a Rat Model of Aluminum Chloride- and D-Galactose-Induced Neurodegeneration
    Article Snippet: The levels of pro-inflammatory cytokines and chemokines in brain samples were determined using a Multi-Analyte ELISArray Kit (Qiagen, Hilden, Germany).

    Article Title: Impact of Epstein-Barr Virus Nuclear Antigen 1 on Neuroinflammation in PARK2 Knockout Mice.
    Article Snippet: Cytokine concentrations in the culture supernatants were then measured using a multi-analyte ELISArray kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions.

    Homogenization:

    Article Title: Antioxidant, antimicrobial and immunomodulatory activities of facial serum formulation containing Melaleuca cajuputi essential oil
    Article Snippet: The percentage of viability was calculated by using the following formula (Lin and Lin, 2020): Viability (%) = (ODtreated cells/ODuntreated cells) × 100 Cytokine assay For the detection of mouse T helper 1 (Th1), T helper 2 (Th2) and T helper 17 (Th17) related cytokines, cytokine assay was performed using a multi-analyte ELISArray kit (QIAGEN, USA) according to the manufacturer’s instructions with cell culture supernatants as samples.

    Article Title: Translation of Data from Animal Models of Cancer to Immunotherapy of Breast Cancer and Chronic Lymphocytic Leukemia
    Article Snippet: Cytokine measurements in vitro/in serum : A Multi-Analyte ELISArray kit (Qiagen, Venlo, The Netherlands) was used to check multiple cytokines in the culture supernatant of BMMSCs or CLL cells alone, or in co-culture.

    Article Title: Protective effects of Centella asiatica extract on spatial memory and learning deficits in animal model of systemic inflammation induced by lipopolysaccharide.
    Article Snippet: On day 21 after the behavioral test, all rats were euthanized and blood was obtained through heart puncture into eDta-coated tubes. samples were centrifuged (6000 g for 15 min at 4 °c) and plasma was collected and stored frozen (–80 °c) until analysis. the plasma cytokines were measured using a Multi-analyte elisarray kit (cat. no. MeR-004a; saBiosciences; Qiagen, inc, Valencia, ca, Usa) according to the manufacturer’s protocol. samples were added to the array, which included the following specific cytokine capture antibodies from the above-mentioned kit: il-1β, il-6, tNf-α, ifN-γ and McP-1. the reaction was measured by changes at 450 and 540 nm on a spectrophotometer.

    Article Title: Anti-Inflammatory Action of Resveratrol in the Central Nervous System in Relation to Glucose Concentration—An In Vitro Study on a Blood–Brain Barrier Model
    Article Snippet: IL-6, IL-8, with the Multi-Analyte ELISArray Kit (Qiagen-Q4Lab, Hilden, Germany; cat. # 336161), according to the instructions of the manufacturer.

    Article Title: Proinflammatory cytokine profiles in prediabetic Saudi patients
    Article Snippet: The Multi-Analyte ELISArray Kit was used to analyze the presence of 12 human cytokines associated with inflammation in plasma samples from prediabetes patients and healthy controls (Qiagen).

    Article Title: Regulation of distinct caspase-8 functions in retinal ganglion cells and astroglia in experimental glaucoma
    Article Snippet: Protein lysates of the retina were obtained by homogenization in a lysis buffer (50 mM HEPES-KOH pH 8.0, 100 mM KCl, 2 mM EDTA, 0.10% NP-40, 2 mM dithiothreitol, 10% glycerol, supplemented with protease and phosphatase inhibitors) as previously described. ( ; ) After protein concentration was determined using a colorimetric Bradford protein assay (BioRad, Hercules, CA), a set of cytokines/chemokines was profiled, as previously described ( ; ), using a Multi-Analyte ELISArray kit (Qiagen, Valencia, CA) with detection sensitivity at low pg/ml levels.

    Article Title: Quercetin as a Therapeutic Option in a Rat Model of Aluminum Chloride- and D-Galactose-Induced Neurodegeneration
    Article Snippet: The levels of pro-inflammatory cytokines and chemokines in brain samples were determined using a Multi-Analyte ELISArray Kit (Qiagen, Hilden, Germany).

    Article Title: Impact of Epstein-Barr Virus Nuclear Antigen 1 on Neuroinflammation in PARK2 Knockout Mice.
    Article Snippet: Cytokine concentrations in the culture supernatants were then measured using a multi-analyte ELISArray kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions.

    Lysis:

    Article Title: Antioxidant, antimicrobial and immunomodulatory activities of facial serum formulation containing Melaleuca cajuputi essential oil
    Article Snippet: The percentage of viability was calculated by using the following formula (Lin and Lin, 2020): Viability (%) = (ODtreated cells/ODuntreated cells) × 100 Cytokine assay For the detection of mouse T helper 1 (Th1), T helper 2 (Th2) and T helper 17 (Th17) related cytokines, cytokine assay was performed using a multi-analyte ELISArray kit (QIAGEN, USA) according to the manufacturer’s instructions with cell culture supernatants as samples.

    Article Title: Translation of Data from Animal Models of Cancer to Immunotherapy of Breast Cancer and Chronic Lymphocytic Leukemia
    Article Snippet: Cytokine measurements in vitro/in serum : A Multi-Analyte ELISArray kit (Qiagen, Venlo, The Netherlands) was used to check multiple cytokines in the culture supernatant of BMMSCs or CLL cells alone, or in co-culture.

    Article Title: Protective effects of Centella asiatica extract on spatial memory and learning deficits in animal model of systemic inflammation induced by lipopolysaccharide.
    Article Snippet: On day 21 after the behavioral test, all rats were euthanized and blood was obtained through heart puncture into eDta-coated tubes. samples were centrifuged (6000 g for 15 min at 4 °c) and plasma was collected and stored frozen (–80 °c) until analysis. the plasma cytokines were measured using a Multi-analyte elisarray kit (cat. no. MeR-004a; saBiosciences; Qiagen, inc, Valencia, ca, Usa) according to the manufacturer’s protocol. samples were added to the array, which included the following specific cytokine capture antibodies from the above-mentioned kit: il-1β, il-6, tNf-α, ifN-γ and McP-1. the reaction was measured by changes at 450 and 540 nm on a spectrophotometer.

    Article Title: Anti-Inflammatory Action of Resveratrol in the Central Nervous System in Relation to Glucose Concentration—An In Vitro Study on a Blood–Brain Barrier Model
    Article Snippet: IL-6, IL-8, with the Multi-Analyte ELISArray Kit (Qiagen-Q4Lab, Hilden, Germany; cat. # 336161), according to the instructions of the manufacturer.

    Article Title: Proinflammatory cytokine profiles in prediabetic Saudi patients
    Article Snippet: The Multi-Analyte ELISArray Kit was used to analyze the presence of 12 human cytokines associated with inflammation in plasma samples from prediabetes patients and healthy controls (Qiagen).

    Article Title: Regulation of distinct caspase-8 functions in retinal ganglion cells and astroglia in experimental glaucoma
    Article Snippet: Protein lysates of the retina were obtained by homogenization in a lysis buffer (50 mM HEPES-KOH pH 8.0, 100 mM KCl, 2 mM EDTA, 0.10% NP-40, 2 mM dithiothreitol, 10% glycerol, supplemented with protease and phosphatase inhibitors) as previously described. ( ; ) After protein concentration was determined using a colorimetric Bradford protein assay (BioRad, Hercules, CA), a set of cytokines/chemokines was profiled, as previously described ( ; ), using a Multi-Analyte ELISArray kit (Qiagen, Valencia, CA) with detection sensitivity at low pg/ml levels.

    Article Title: Quercetin as a Therapeutic Option in a Rat Model of Aluminum Chloride- and D-Galactose-Induced Neurodegeneration
    Article Snippet: The levels of pro-inflammatory cytokines and chemokines in brain samples were determined using a Multi-Analyte ELISArray Kit (Qiagen, Hilden, Germany).

    Article Title: Impact of Epstein-Barr Virus Nuclear Antigen 1 on Neuroinflammation in PARK2 Knockout Mice.
    Article Snippet: Cytokine concentrations in the culture supernatants were then measured using a multi-analyte ELISArray kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions.

    Protein Concentration:

    Article Title: Antioxidant, antimicrobial and immunomodulatory activities of facial serum formulation containing Melaleuca cajuputi essential oil
    Article Snippet: The percentage of viability was calculated by using the following formula (Lin and Lin, 2020): Viability (%) = (ODtreated cells/ODuntreated cells) × 100 Cytokine assay For the detection of mouse T helper 1 (Th1), T helper 2 (Th2) and T helper 17 (Th17) related cytokines, cytokine assay was performed using a multi-analyte ELISArray kit (QIAGEN, USA) according to the manufacturer’s instructions with cell culture supernatants as samples.

    Article Title: Translation of Data from Animal Models of Cancer to Immunotherapy of Breast Cancer and Chronic Lymphocytic Leukemia
    Article Snippet: Cytokine measurements in vitro/in serum : A Multi-Analyte ELISArray kit (Qiagen, Venlo, The Netherlands) was used to check multiple cytokines in the culture supernatant of BMMSCs or CLL cells alone, or in co-culture.

    Article Title: Protective effects of Centella asiatica extract on spatial memory and learning deficits in animal model of systemic inflammation induced by lipopolysaccharide.
    Article Snippet: On day 21 after the behavioral test, all rats were euthanized and blood was obtained through heart puncture into eDta-coated tubes. samples were centrifuged (6000 g for 15 min at 4 °c) and plasma was collected and stored frozen (–80 °c) until analysis. the plasma cytokines were measured using a Multi-analyte elisarray kit (cat. no. MeR-004a; saBiosciences; Qiagen, inc, Valencia, ca, Usa) according to the manufacturer’s protocol. samples were added to the array, which included the following specific cytokine capture antibodies from the above-mentioned kit: il-1β, il-6, tNf-α, ifN-γ and McP-1. the reaction was measured by changes at 450 and 540 nm on a spectrophotometer.

    Article Title: Anti-Inflammatory Action of Resveratrol in the Central Nervous System in Relation to Glucose Concentration—An In Vitro Study on a Blood–Brain Barrier Model
    Article Snippet: IL-6, IL-8, with the Multi-Analyte ELISArray Kit (Qiagen-Q4Lab, Hilden, Germany; cat. # 336161), according to the instructions of the manufacturer.

    Article Title: Proinflammatory cytokine profiles in prediabetic Saudi patients
    Article Snippet: The Multi-Analyte ELISArray Kit was used to analyze the presence of 12 human cytokines associated with inflammation in plasma samples from prediabetes patients and healthy controls (Qiagen).

    Article Title: Regulation of distinct caspase-8 functions in retinal ganglion cells and astroglia in experimental glaucoma
    Article Snippet: Protein lysates of the retina were obtained by homogenization in a lysis buffer (50 mM HEPES-KOH pH 8.0, 100 mM KCl, 2 mM EDTA, 0.10% NP-40, 2 mM dithiothreitol, 10% glycerol, supplemented with protease and phosphatase inhibitors) as previously described. ( ; ) After protein concentration was determined using a colorimetric Bradford protein assay (BioRad, Hercules, CA), a set of cytokines/chemokines was profiled, as previously described ( ; ), using a Multi-Analyte ELISArray kit (Qiagen, Valencia, CA) with detection sensitivity at low pg/ml levels.

    Article Title: Quercetin as a Therapeutic Option in a Rat Model of Aluminum Chloride- and D-Galactose-Induced Neurodegeneration
    Article Snippet: The levels of pro-inflammatory cytokines and chemokines in brain samples were determined using a Multi-Analyte ELISArray Kit (Qiagen, Hilden, Germany).

    Article Title: Impact of Epstein-Barr Virus Nuclear Antigen 1 on Neuroinflammation in PARK2 Knockout Mice.
    Article Snippet: Cytokine concentrations in the culture supernatants were then measured using a multi-analyte ELISArray kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions.

    Bradford Protein Assay:

    Article Title: Antioxidant, antimicrobial and immunomodulatory activities of facial serum formulation containing Melaleuca cajuputi essential oil
    Article Snippet: The percentage of viability was calculated by using the following formula (Lin and Lin, 2020): Viability (%) = (ODtreated cells/ODuntreated cells) × 100 Cytokine assay For the detection of mouse T helper 1 (Th1), T helper 2 (Th2) and T helper 17 (Th17) related cytokines, cytokine assay was performed using a multi-analyte ELISArray kit (QIAGEN, USA) according to the manufacturer’s instructions with cell culture supernatants as samples.

    Article Title: Translation of Data from Animal Models of Cancer to Immunotherapy of Breast Cancer and Chronic Lymphocytic Leukemia
    Article Snippet: Cytokine measurements in vitro/in serum : A Multi-Analyte ELISArray kit (Qiagen, Venlo, The Netherlands) was used to check multiple cytokines in the culture supernatant of BMMSCs or CLL cells alone, or in co-culture.

    Article Title: Protective effects of Centella asiatica extract on spatial memory and learning deficits in animal model of systemic inflammation induced by lipopolysaccharide.
    Article Snippet: On day 21 after the behavioral test, all rats were euthanized and blood was obtained through heart puncture into eDta-coated tubes. samples were centrifuged (6000 g for 15 min at 4 °c) and plasma was collected and stored frozen (–80 °c) until analysis. the plasma cytokines were measured using a Multi-analyte elisarray kit (cat. no. MeR-004a; saBiosciences; Qiagen, inc, Valencia, ca, Usa) according to the manufacturer’s protocol. samples were added to the array, which included the following specific cytokine capture antibodies from the above-mentioned kit: il-1β, il-6, tNf-α, ifN-γ and McP-1. the reaction was measured by changes at 450 and 540 nm on a spectrophotometer.

    Article Title: Anti-Inflammatory Action of Resveratrol in the Central Nervous System in Relation to Glucose Concentration—An In Vitro Study on a Blood–Brain Barrier Model
    Article Snippet: IL-6, IL-8, with the Multi-Analyte ELISArray Kit (Qiagen-Q4Lab, Hilden, Germany; cat. # 336161), according to the instructions of the manufacturer.

    Article Title: Proinflammatory cytokine profiles in prediabetic Saudi patients
    Article Snippet: The Multi-Analyte ELISArray Kit was used to analyze the presence of 12 human cytokines associated with inflammation in plasma samples from prediabetes patients and healthy controls (Qiagen).

    Article Title: Regulation of distinct caspase-8 functions in retinal ganglion cells and astroglia in experimental glaucoma
    Article Snippet: Protein lysates of the retina were obtained by homogenization in a lysis buffer (50 mM HEPES-KOH pH 8.0, 100 mM KCl, 2 mM EDTA, 0.10% NP-40, 2 mM dithiothreitol, 10% glycerol, supplemented with protease and phosphatase inhibitors) as previously described. ( ; ) After protein concentration was determined using a colorimetric Bradford protein assay (BioRad, Hercules, CA), a set of cytokines/chemokines was profiled, as previously described ( ; ), using a Multi-Analyte ELISArray kit (Qiagen, Valencia, CA) with detection sensitivity at low pg/ml levels.

    Article Title: Quercetin as a Therapeutic Option in a Rat Model of Aluminum Chloride- and D-Galactose-Induced Neurodegeneration
    Article Snippet: The levels of pro-inflammatory cytokines and chemokines in brain samples were determined using a Multi-Analyte ELISArray Kit (Qiagen, Hilden, Germany).

    Article Title: Impact of Epstein-Barr Virus Nuclear Antigen 1 on Neuroinflammation in PARK2 Knockout Mice.
    Article Snippet: Cytokine concentrations in the culture supernatants were then measured using a multi-analyte ELISArray kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions.

    Clinical Proteomics:

    Article Title: Antioxidant, antimicrobial and immunomodulatory activities of facial serum formulation containing Melaleuca cajuputi essential oil
    Article Snippet: The percentage of viability was calculated by using the following formula (Lin and Lin, 2020): Viability (%) = (ODtreated cells/ODuntreated cells) × 100 Cytokine assay For the detection of mouse T helper 1 (Th1), T helper 2 (Th2) and T helper 17 (Th17) related cytokines, cytokine assay was performed using a multi-analyte ELISArray kit (QIAGEN, USA) according to the manufacturer’s instructions with cell culture supernatants as samples.

    Article Title: Translation of Data from Animal Models of Cancer to Immunotherapy of Breast Cancer and Chronic Lymphocytic Leukemia
    Article Snippet: Cytokine measurements in vitro/in serum : A Multi-Analyte ELISArray kit (Qiagen, Venlo, The Netherlands) was used to check multiple cytokines in the culture supernatant of BMMSCs or CLL cells alone, or in co-culture.

    Article Title: Protective effects of Centella asiatica extract on spatial memory and learning deficits in animal model of systemic inflammation induced by lipopolysaccharide.
    Article Snippet: On day 21 after the behavioral test, all rats were euthanized and blood was obtained through heart puncture into eDta-coated tubes. samples were centrifuged (6000 g for 15 min at 4 °c) and plasma was collected and stored frozen (–80 °c) until analysis. the plasma cytokines were measured using a Multi-analyte elisarray kit (cat. no. MeR-004a; saBiosciences; Qiagen, inc, Valencia, ca, Usa) according to the manufacturer’s protocol. samples were added to the array, which included the following specific cytokine capture antibodies from the above-mentioned kit: il-1β, il-6, tNf-α, ifN-γ and McP-1. the reaction was measured by changes at 450 and 540 nm on a spectrophotometer.

    Article Title: Anti-Inflammatory Action of Resveratrol in the Central Nervous System in Relation to Glucose Concentration—An In Vitro Study on a Blood–Brain Barrier Model
    Article Snippet: IL-6, IL-8, with the Multi-Analyte ELISArray Kit (Qiagen-Q4Lab, Hilden, Germany; cat. # 336161), according to the instructions of the manufacturer.

    Article Title: Proinflammatory cytokine profiles in prediabetic Saudi patients
    Article Snippet: The Multi-Analyte ELISArray Kit was used to analyze the presence of 12 human cytokines associated with inflammation in plasma samples from prediabetes patients and healthy controls (Qiagen).

    Article Title: Regulation of distinct caspase-8 functions in retinal ganglion cells and astroglia in experimental glaucoma
    Article Snippet: Protein lysates of the retina were obtained by homogenization in a lysis buffer (50 mM HEPES-KOH pH 8.0, 100 mM KCl, 2 mM EDTA, 0.10% NP-40, 2 mM dithiothreitol, 10% glycerol, supplemented with protease and phosphatase inhibitors) as previously described. ( ; ) After protein concentration was determined using a colorimetric Bradford protein assay (BioRad, Hercules, CA), a set of cytokines/chemokines was profiled, as previously described ( ; ), using a Multi-Analyte ELISArray kit (Qiagen, Valencia, CA) with detection sensitivity at low pg/ml levels.

    Article Title: Quercetin as a Therapeutic Option in a Rat Model of Aluminum Chloride- and D-Galactose-Induced Neurodegeneration
    Article Snippet: The levels of pro-inflammatory cytokines and chemokines in brain samples were determined using a Multi-Analyte ELISArray Kit (Qiagen, Hilden, Germany).

    Article Title: Impact of Epstein-Barr Virus Nuclear Antigen 1 on Neuroinflammation in PARK2 Knockout Mice.
    Article Snippet: Cytokine concentrations in the culture supernatants were then measured using a multi-analyte ELISArray kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions.

    Spectrophotometry:

    Article Title: Antioxidant, antimicrobial and immunomodulatory activities of facial serum formulation containing Melaleuca cajuputi essential oil
    Article Snippet: The percentage of viability was calculated by using the following formula (Lin and Lin, 2020): Viability (%) = (ODtreated cells/ODuntreated cells) × 100 Cytokine assay For the detection of mouse T helper 1 (Th1), T helper 2 (Th2) and T helper 17 (Th17) related cytokines, cytokine assay was performed using a multi-analyte ELISArray kit (QIAGEN, USA) according to the manufacturer’s instructions with cell culture supernatants as samples.

    Article Title: Translation of Data from Animal Models of Cancer to Immunotherapy of Breast Cancer and Chronic Lymphocytic Leukemia
    Article Snippet: Cytokine measurements in vitro/in serum : A Multi-Analyte ELISArray kit (Qiagen, Venlo, The Netherlands) was used to check multiple cytokines in the culture supernatant of BMMSCs or CLL cells alone, or in co-culture.

    Article Title: Protective effects of Centella asiatica extract on spatial memory and learning deficits in animal model of systemic inflammation induced by lipopolysaccharide.
    Article Snippet: On day 21 after the behavioral test, all rats were euthanized and blood was obtained through heart puncture into eDta-coated tubes. samples were centrifuged (6000 g for 15 min at 4 °c) and plasma was collected and stored frozen (–80 °c) until analysis. the plasma cytokines were measured using a Multi-analyte elisarray kit (cat. no. MeR-004a; saBiosciences; Qiagen, inc, Valencia, ca, Usa) according to the manufacturer’s protocol. samples were added to the array, which included the following specific cytokine capture antibodies from the above-mentioned kit: il-1β, il-6, tNf-α, ifN-γ and McP-1. the reaction was measured by changes at 450 and 540 nm on a spectrophotometer.

    Article Title: Anti-Inflammatory Action of Resveratrol in the Central Nervous System in Relation to Glucose Concentration—An In Vitro Study on a Blood–Brain Barrier Model
    Article Snippet: IL-6, IL-8, with the Multi-Analyte ELISArray Kit (Qiagen-Q4Lab, Hilden, Germany; cat. # 336161), according to the instructions of the manufacturer.

    Article Title: Proinflammatory cytokine profiles in prediabetic Saudi patients
    Article Snippet: The Multi-Analyte ELISArray Kit was used to analyze the presence of 12 human cytokines associated with inflammation in plasma samples from prediabetes patients and healthy controls (Qiagen).

    Article Title: Regulation of distinct caspase-8 functions in retinal ganglion cells and astroglia in experimental glaucoma
    Article Snippet: Protein lysates of the retina were obtained by homogenization in a lysis buffer (50 mM HEPES-KOH pH 8.0, 100 mM KCl, 2 mM EDTA, 0.10% NP-40, 2 mM dithiothreitol, 10% glycerol, supplemented with protease and phosphatase inhibitors) as previously described. ( ; ) After protein concentration was determined using a colorimetric Bradford protein assay (BioRad, Hercules, CA), a set of cytokines/chemokines was profiled, as previously described ( ; ), using a Multi-Analyte ELISArray kit (Qiagen, Valencia, CA) with detection sensitivity at low pg/ml levels.

    Article Title: Quercetin as a Therapeutic Option in a Rat Model of Aluminum Chloride- and D-Galactose-Induced Neurodegeneration
    Article Snippet: The levels of pro-inflammatory cytokines and chemokines in brain samples were determined using a Multi-Analyte ELISArray Kit (Qiagen, Hilden, Germany).

    Article Title: Impact of Epstein-Barr Virus Nuclear Antigen 1 on Neuroinflammation in PARK2 Knockout Mice.
    Article Snippet: Cytokine concentrations in the culture supernatants were then measured using a multi-analyte ELISArray kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions.



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    The effect of carbon black (CB), urban dust (UD), and nanoparticulate carbon black (NPCB) on cell membrane damage (( A ); lactate dehydrogenase (LDH) release), oxidative stress ( B ), DNA damage ( C ), and phagocytosis ( D ) in human monocytes (M), and monocyte-derived macrophages (MDM). The inflammatory response (TNFα; ( E )) to lipopolysaccharide (100 ng/mL for 24 h) is also included ( F ). All PMs were used at 100 µg/mL and the incubation time was 24 h. Cell transition was induced by phorbol-12-myristate 13-acetate (PMA; 100 nM) applied for 72 h. LDH was measured with CyQUANT LDH Cytotoxicity Assay kit, DNA ploidy was quantified using propidium iodide staining, intracellular oxidative stress was measured using 5-(and-6)-Carboxy-2′,7′-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA) while phagocytosis was assessed with fluorescent beads (Cayman Phagocytosis kit). Fluorescence was quantified with flow cytometry. TNFα was measured in the culture medium using a Multi-Analyte Inflammatory <t>Cytokine</t> <t>ELISArray</t> Kit (Quiagen, Wroclaw, Poland). Data are means ± SD of 6–10 assays. * p < 0.05; ** p < 0.01 for comparisons with the corresponding control cells; ^^ p < 0.01 for comparisons with corresponding naive cells; # p < 0.05 for comparisons with LPS-treated cells.
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    The effect of carbon black (CB), urban dust (UD), and nanoparticulate carbon black (NPCB) on cell membrane damage (( A ); lactate dehydrogenase (LDH) release), oxidative stress ( B ), DNA damage ( C ), and phagocytosis ( D ) in human monocytes (M), and monocyte-derived macrophages (MDM). The inflammatory response (TNFα; ( E )) to lipopolysaccharide (100 ng/mL for 24 h) is also included ( F ). All PMs were used at 100 µg/mL and the incubation time was 24 h. Cell transition was induced by phorbol-12-myristate 13-acetate (PMA; 100 nM) applied for 72 h. LDH was measured with CyQUANT LDH Cytotoxicity Assay kit, DNA ploidy was quantified using propidium iodide staining, intracellular oxidative stress was measured using 5-(and-6)-Carboxy-2′,7′-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA) while phagocytosis was assessed with fluorescent beads (Cayman Phagocytosis kit). Fluorescence was quantified with flow cytometry. TNFα was measured in the culture medium using a Multi-Analyte Inflammatory <t>Cytokine</t> <t>ELISArray</t> Kit (Quiagen, Wroclaw, Poland). Data are means ± SD of 6–10 assays. * p < 0.05; ** p < 0.01 for comparisons with the corresponding control cells; ^^ p < 0.01 for comparisons with corresponding naive cells; # p < 0.05 for comparisons with LPS-treated cells.
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    The effect of carbon black (CB), urban dust (UD), and nanoparticulate carbon black (NPCB) on cell membrane damage (( A ); lactate dehydrogenase (LDH) release), oxidative stress ( B ), DNA damage ( C ), and phagocytosis ( D ) in human monocytes (M), and monocyte-derived macrophages (MDM). The inflammatory response (TNFα; ( E )) to lipopolysaccharide (100 ng/mL for 24 h) is also included ( F ). All PMs were used at 100 µg/mL and the incubation time was 24 h. Cell transition was induced by phorbol-12-myristate 13-acetate (PMA; 100 nM) applied for 72 h. LDH was measured with CyQUANT LDH Cytotoxicity Assay kit, DNA ploidy was quantified using propidium iodide staining, intracellular oxidative stress was measured using 5-(and-6)-Carboxy-2′,7′-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA) while phagocytosis was assessed with fluorescent beads (Cayman Phagocytosis kit). Fluorescence was quantified with flow cytometry. TNFα was measured in the culture medium using a Multi-Analyte Inflammatory Cytokine ELISArray Kit (Quiagen, Wroclaw, Poland). Data are means ± SD of 6–10 assays. * p < 0.05; ** p < 0.01 for comparisons with the corresponding control cells; ^^ p < 0.01 for comparisons with corresponding naive cells; # p < 0.05 for comparisons with LPS-treated cells.

    Journal: Cells

    Article Title: Signalling Pathways of Inflammation and Cancer in Human Mononuclear Cells: Effect of Nanoparticle Air Pollutants

    doi: 10.3390/cells13161367

    Figure Lengend Snippet: The effect of carbon black (CB), urban dust (UD), and nanoparticulate carbon black (NPCB) on cell membrane damage (( A ); lactate dehydrogenase (LDH) release), oxidative stress ( B ), DNA damage ( C ), and phagocytosis ( D ) in human monocytes (M), and monocyte-derived macrophages (MDM). The inflammatory response (TNFα; ( E )) to lipopolysaccharide (100 ng/mL for 24 h) is also included ( F ). All PMs were used at 100 µg/mL and the incubation time was 24 h. Cell transition was induced by phorbol-12-myristate 13-acetate (PMA; 100 nM) applied for 72 h. LDH was measured with CyQUANT LDH Cytotoxicity Assay kit, DNA ploidy was quantified using propidium iodide staining, intracellular oxidative stress was measured using 5-(and-6)-Carboxy-2′,7′-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA) while phagocytosis was assessed with fluorescent beads (Cayman Phagocytosis kit). Fluorescence was quantified with flow cytometry. TNFα was measured in the culture medium using a Multi-Analyte Inflammatory Cytokine ELISArray Kit (Quiagen, Wroclaw, Poland). Data are means ± SD of 6–10 assays. * p < 0.05; ** p < 0.01 for comparisons with the corresponding control cells; ^^ p < 0.01 for comparisons with corresponding naive cells; # p < 0.05 for comparisons with LPS-treated cells.

    Article Snippet: TNFα was quantified in the culture medium of M and MDM with the Multi-Analyte Inflammatory Cytokine ELISArray Kit (Qiagen, Manchester, UK).

    Techniques: Membrane, Derivative Assay, Incubation, CyQUANT Assay, LDH Cytotoxicity Assay, Staining, Fluorescence, Flow Cytometry, Control